polylinker site

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• Polylinker — Ein Polylinker oder auch Multiple Cloning Site (MCS) ist ein künstlich geschaffenes DNA Oligonukleotid aus rund 50bp, welches in ein Plasmid eingesetzt wird und dessen Sequenz direkt hintereinander verschiedene Restriktionsschnittstellen für… …   Deutsch Wikipedia

• polylinker — An inserted sequence of DNA in recombinant DNA vectors consisting of a cluster of numerous restriction endonuclease sites unique in the plasmid; also called restriction site bank and polycloning site …   Medical dictionary

• polylinker — A synthetic segment of DNA, designed to include a number of different restriction endonuclease sites. When ligated to a DNA fragment that is to be cloned, this enables a wide choice of restriction endonucleases to be used for the cloning process …   Glossary of Biotechnology

• Multiple cloning site — A multiple cloning site (MCS), also called a polylinker, is a short segment of DNA which contains many (up to 20) restriction sites a standard feature of engineered plasmids.[1] Restriction sites within an MCS are typically unique, occurring only …   Wikipedia

• Multiple Cloning Site — Ein Polylinker oder eine Multiple Cloning Site (MCS) ist ein DNA Abschnitt in einem Vektor, dessen Sequenz direkt hintereinander verschiedene Schnittstellen für Restriktionsendonukleasen enthält. Diese ermöglicht ein flexibles Klonieren, da aus… …   Deutsch Wikipedia

• multiple cloning site — Abbreviation: MCS See: polylinker …   Glossary of Biotechnology

• polycloning site — See: polylinker …   Glossary of Biotechnology

• Vector de clonación — Los vectores de clonación son moléculas transportadoras que transfieren y replican fragmentos de ADN que llevan insertados. Para que sirva de vector, una molécula debe ser capaz de replicarse junto con el fragmento de ADN que transporta. También… …   Wikipedia Español

• Restriction enzyme — Glossary Restriction …   Wikipedia

• Plasmid — Figure 1: Illustration of a bacterium with plasmid enclosed showing chromosomal DNA and plasmids. In microbiology and genetics, a plasmid is a DNA molecule that is separate from, and can replicate independently of, the chromosomal DNA …   Wikipedia

• Restriction digest — A restriction digest is a procedure used in molecular biology to prepare DNA for analysis or other processing. It is sometimes termed DNA fragmentation (this term is used for other procedures as well). Hartl and Jones describe it this way: This… …   Wikipedia